anti human β actin rabbit cell signaling technologies  (Cell Signaling Technology Inc)

Journal: BMC cancer

Article Title: The combination of poly(ADP-ribose) polymerase inhibitor and statin inhibits the proliferation of human castration-resistant and taxane-resistant prostate cancer cells in vitro and in vivo.

doi: 10.1186/s12885-025-13895-6

Figure Lengend Snippet: Fig. 1 Effect of simvastatin on DNA repair gene expression in prostate cancer and prostate stromal cells (PrSC). PC-3, 22Rv1, LNCaP-LA, LNCaP, and PrSC cells were incubated with the medium containing 10% FBS for 24 h, and the medium was switched to the indicated concentration of simvastatin in the medium containing 10% FBS. After 48 h, the total RNA (A, C) and total protein (B, D) were collected. (A) Comparison of mRNA expression levels of each gene in each cell without simvastatin. mRNA expression of BRCA1, BRCA2, RAD51, FANCD2, FANCG, FANCA, BARD1, RFC3, RFC4, and RFC5 was evalu ated by performing real-time polymerase chain reaction tests, and the relative quantitative volume (RQV) was calculated by comparing the expression of β-actin. Values are expressed as mean ± standard deviation (SD) (n = 3). *P < 0.05 vs. the other cells. (B) Comparison of protein levels of BRCA1 and RAD51 in each cell without simvastatin. The protein expression of BRCA1 and RAD51 was evaluated by western blotting. A representative experiment is shown, which was repeated three times with similar results. (C) Comparison of mRNA expression levels of each gene in each cell after treatment of simvastatin. mRNA expression of BRCA1, BRCA2, RAD51, FANCD2, FANCG, FANCA, BARD1, RFC3, RFC4, and RFC5 was evaluated by performing real-time polymerase chain reaction tests, and the relative quantitative volume (RQV) was calculated by comparing the expression of β-actin. Values are expressed as mean ± standard deviation (SD) (n = 3). *P < 0.05 vs. 0 µM of each cell. (D) Comparison of BRCA1 protein levels in each cell after treatment of simvastatin. The protein expression of BRCA1 was evaluated by western blotting. A representative experiment is shown, which was repeated three times with similar results

Article Snippet: Rabbit anti-BRCA1 polyclonal antibody, Rabbit anti-RAD51 Recombinase (RAD51) monoclonal antibody, mouse anti-BARD1 monoclonal antibody, rabbit anti-FANCA monoclonal antibody, rabbit anti-γH2AX monoclonal antibody and rabbit anti-human β-actin monoclonal antibody were purchased from Santa Cruz (Dallas, USA), Cell Signaling (Beverly, USA), Cell Signaling (Beverly, USA), Cell Signaling (Beverly, USA), Cell Signaling (Beverly, USA) and A&G Pharmaceutical (Columbia, USA), respectively.

Techniques: Gene Expression, Incubation, Concentration Assay, Comparison, Expressing, Real-time Polymerase Chain Reaction, Standard Deviation, Western Blot

Journal: BMC cancer

Article Title: The combination of poly(ADP-ribose) polymerase inhibitor and statin inhibits the proliferation of human castration-resistant and taxane-resistant prostate cancer cells in vitro and in vivo.

doi: 10.1186/s12885-025-13895-6

Figure Lengend Snippet: Fig. 5 Effect of combination therapy with simvastatin and olaparib on taxane-resistant prostate cancer cells. A and B. Comparison of DNA repair gene expression in 22Rv1 and 22Rv1-CR cells. A. mRNA expression of BRCA1, BRCA2, RAD51, BARD1, and FANCA was evaluated by RT-PCR, and the relative quantitative volume was calculated by comparing the expression of β-actin. Values are expressed as means ± standard deviations (SD) (n = 3). *P < 0.05 vs. 22Rv1 cells. B. Protein expression of BRCA1, RAD51, BARD1, and FANCA was evaluated using western blotting. A representative experiment is shown, which was repeated three times with similar results. C and D. Cells were incubated in medium containing various concentrations of simvastatin. After 48 h, the total RNA (C) and total protein (D) were collected. A. mRNA expression of BRCA1, BRCA2, RAD51, BARD1, and FANCA was evaluated by RT-PCR, and the relative quantitative volume was calculated by comparing the expression of β-actin. Values are expressed as mean ± standard deviation (SD) (n = 3). *P < 0.05 vs. 0 µM of each gene. D. Protein expression of BRCA1 and FANCA was evaluated by western blotting. A representative experiment is shown, which was repeated three times with similar results. E and F. Cells were incubated in the medium. After 48 h, the cells were cultured in a medium containing olaparib (10 µM) with or without simvastatin (5 µM). After 96 h, the number of viable cells was evaluated using the MTS assay (E) and cell counts (F). *P < 0.05. Sim; simvastatin, Ola; olaparib

Article Snippet: Rabbit anti-BRCA1 polyclonal antibody, Rabbit anti-RAD51 Recombinase (RAD51) monoclonal antibody, mouse anti-BARD1 monoclonal antibody, rabbit anti-FANCA monoclonal antibody, rabbit anti-γH2AX monoclonal antibody and rabbit anti-human β-actin monoclonal antibody were purchased from Santa Cruz (Dallas, USA), Cell Signaling (Beverly, USA), Cell Signaling (Beverly, USA), Cell Signaling (Beverly, USA), Cell Signaling (Beverly, USA) and A&G Pharmaceutical (Columbia, USA), respectively.

Techniques: Comparison, Gene Expression, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Incubation, Standard Deviation, Cell Culture, MTS Assay